Bioprocessing is an important tool in industrial biotechnology, from the production of therapeutic proteins and vaccines in the pharmaceutical industry to food and feed engineering, up to the sustainable production of fuels, chemicals, and polymers. Each application has its individual challenges and requirements, that must be considered for that specific process. All aim on the generation of the product in consistently high quality, sufficient yields, and in a fast, cost-efficient manner. Successful bioprocessing is complex and depends on many factors. From the selection of the expression host, media composition, the proper cultivation system and cultivation strategy, adequate downstream processes, up to the development of a robust tech transfer.
In this webinar we will address general and day-to-day challenges in the bioprocessing workflow from shake flask to production and discuss possible solutions. We will initially look on common challenges in reactor set-ups and configurations. What vessel and impeller type is the appropriate one for different culture demands (e.g. shear sensitivity, high oxygen demands), what process parameters control is essential and how the integration of additional analytical tools can help to improve process stability. We will address frequent challenges in cultivation, e.g. how to produce reproducible inoculums in shake flasks, how to prevent contamination in a bioreactor, during transfer or sampling and how to cope with difficulties in cultivation in the bioreactor, e.g. foaming or viscous solutions. We will further have a look on the different steps of a process and discuss optimization factors and measures, how to improve yields for example by measuring the kLa or choosing the right process strategy. Once a process is established, the aim is to transfer the process step-wise to larger volumes without losing efficiency and product quality. Successful scale-up poses several challenges, including the proper tech transfer of the operating conditions. Which scale-up strategy helps to overcome these challenges best, and what digital tools are available to ease scale transition? Finally, another critical bioprocessing bottleneck is the harvesting process. We will have a short glance on harvesting optimization in the end and evaluate, how to handle best high volumes by keeping downstream productivity.
So register now, if you want to have answers to frequently asked questions in bioprocessing and understand how to cope with challenges in bioprocessing from shake flask to bioreactor.
Key Learning Objectives
- Get answers to frequently asked questions in bioprocessing.
- Learn how to define and optimize important parameters to improve culture results.
- Get hands-on advice to optimize each step of your process.
