With timelines shortening and biologics pipelines growing, a need to generate robust process development methods quickly is essential. An Upstream High-Throughput Biologics Development (HTBD) strategy making use of a Tecan liquid-handling system with Design-of-Experiment (DOE) studies has been developed and implemented at the Devens site of Bristol Myers Squibb to meet this demand. However, we discovered that our previous metabolite analyzer greatly held back the potential of our high-throughput experiments, costing us what no-one can afford: time.
This presentation will be a discussion of the Upstream HTBD strategy at BMS - Devens, the success of this strategy, and how moving from a low-throughput to a high-throughput metabolite analyzer was instrumental for this success. This move effectively increased the number of unique experimental conditions evaluated in a two week period from 96 to 288.
A comparison between the previous and current analyzers will be made, depicting the importance of streamlining HT metabolite sampling workflows to prevent crashes, broken equipment, required replacements/calibrations, downtime, loss of data, and increase throughput. The improvements provided by our current analyzer to our workflow have made for a much more fluid high-throughput experience.
So, if you’re looking for a new approach to improve the efficiency of your DOE designs and high-throughput cell culture workflows, then please register.
Key Learning Objectives
- How to design large DOE upstream process development studies utilizing JMP and a liquid handler
- How unoptimized workflows can negatively impact upstream HT DOE designs and throughput
- How an efficient HT metabolite analyzer can streamline these workflows to significantly increase DOE design space and throughput
Audience
- Biomanufacturing
- Bioprocess Control
- Bioprocess Analytics
- Director
- Manager
- Technician
- Scientist
- Compliance
- Engineering
- Quality Assurance
- Quality Control
- Regulatory Affairs
- Research and Development
- Validation
- Release Testing
- Upstream Process Development
- Upstream Process Manufacturing
